Journal: Cell Reports Medicine

Article Title: p63 controls metabolic activation of hepatic stellate cells and fibrosis via an HER2-ACC1 pathway

doi: 10.1016/j.xcrm.2024.101401

Figure Lengend Snippet: p63 is upregulated in activated primary cultures of stellate cells from murine models or human patients (A) Levels of p63 mRNA in PmHSCs isolated from mice fed an MCDD for 6 weeks (n = 3) or (as a control) a standard diet (SD) for 6 weeks (n = 3). (B and C) Mice fed a CDHFD (n = 4) or SD for 52 weeks (n = 4) (B), and (C) treated with carbon tetrachloride (0.6 mL/kg i.p.) (n = 3) or (as a control) with vehicle (n = 4) once per week for 6 weeks. (D) mRNA expression of p63 and fibrogenesis markers in PrHSCs activated in culture (n = 4–8). (E) Expression of fibrotic markers and p63 in PhHSCs from a donor following the administration of 8 ng/mL TGF-β1 for 24 h (n = 8). (F) mRNA expression of fibrotic markers, total p63, TAp63, ΔNp63, and TAp63 protein levels in human LX-2 (n = 5–6) treated with TGF-β1 for 0, 12, or 24 h (n = 5–6). Hypoxanthine phosphoribosyltransferase (HPRT) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) were used to normalize mRNA and protein levels. Data are mean ± SEM. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.

Article Snippet: For plasmid transfection in human LX-2 cells, we used a DNA plasmid containing the sequence necessary to increase the expression of pTAp63 (27008, Addgene), ΔNp63 (26979, Addgene), HER2 (16257, Addgene) and an empty plasmid as control.

Techniques: Isolation, Control, Expressing

Journal: Cell Reports Medicine

Article Title: p63 controls metabolic activation of hepatic stellate cells and fibrosis via an HER2-ACC1 pathway

doi: 10.1016/j.xcrm.2024.101401

Figure Lengend Snippet: Overexpression of TAp63 activates human LX-2 cells (A) mRNA expression of TAp63 and ΔNp63 (n = 4–6), and protein levels of TAp63 (n = 5–6) in LX-2 cell after overexpressing TAp63. (B) OCR. (C and D) ECAR (C) and (D) basal metabolism in LX-2 cells overexpressing TAp63 (n = 3). Arrows indicate the time point at which metabolic modulators (Oligo, FCCP, Rot/AA, and 2-DG) were added to the assay. (E) Expression of markers of lipid metabolism (n = 4–6). (F and G) Protein levels of ACC and p-ACC (n = 4) (F), and (G) mRNA levels of fibrogenic markers (n = 4). HPRT and GAPDH were used to normalize mRNA and protein levels. Data are mean ± SEM. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001. See also Figure S4 .

Article Snippet: For plasmid transfection in human LX-2 cells, we used a DNA plasmid containing the sequence necessary to increase the expression of pTAp63 (27008, Addgene), ΔNp63 (26979, Addgene), HER2 (16257, Addgene) and an empty plasmid as control.

Techniques: Over Expression, Expressing

Journal: Brain Sciences

Article Title: Prevotella histicola Transplantation Ameliorates Cognitive Impairment and Decreases Oxidative Stress in Vascular Dementia Rats

doi: 10.3390/brainsci13081136

Figure Lengend Snippet: Prevotella histicola transplantation improves neurological behavior of VaD model rats. ( A ) The latency, ( B ) exploration distance, ( C ) times passed through platform, ( D ) time in target quadrant, ( E ) time to target and ( F ) swimming speed of rats in different groups (n = 30 per group). Results are shown as mean ± SD. *** p < 0.001 versus the sham group; ### p < 0.001 versus the 2VO group.

Article Snippet: Prevotella histicola (DSMZ, Braunschweig, Germany) was fully dispersed into sterile water (10 10 cfu/g).

Techniques: Transplantation Assay

Journal: Brain Sciences

Article Title: Prevotella histicola Transplantation Ameliorates Cognitive Impairment and Decreases Oxidative Stress in Vascular Dementia Rats

doi: 10.3390/brainsci13081136

Figure Lengend Snippet: Prevotella histicola transplantation affects hippocampal pathological morphology and synapse-associated protein expression in VaD model rats. ( A ) Images reflecting neurons in the hippocampus tissue of the rats across the groups were detected via Nissl staining, bar = 50 μm (n = 6 per group). ( B ) Quantification of the Nissl-positive cells are shown as a bar chart (n = 6 per group). The expression of MAP2 ( C , D ), SYP ( C , E ) and PSD-95 ( C , F ) in hippocampus tissue from the indicated group were detected by Western blot assay; β-actin served as a loading control (n = 6 per group). Results are shown as mean ± SD. *** p < 0.001 versus the sham group; ## p < 0.01, ### p < 0.001 versus the 2VO group.

Article Snippet: Prevotella histicola (DSMZ, Braunschweig, Germany) was fully dispersed into sterile water (10 10 cfu/g).

Techniques: Transplantation Assay, Expressing, Staining, Western Blot, Control

Journal: Brain Sciences

Article Title: Prevotella histicola Transplantation Ameliorates Cognitive Impairment and Decreases Oxidative Stress in Vascular Dementia Rats

doi: 10.3390/brainsci13081136

Figure Lengend Snippet: Prevotella histicola transplantation inhibits the apoptosis of hippocampal neurons in VaD model rats. ( A ) Representative bands of Bcl-2, Bax and cleaved caspase-3 protein expression within the hippocampi of the rats across the groups. β-actin served as a loading control (n = 6). Quantification for ( B ) Bcl-2, ( C ) Bax and ( D ) cleaved caspase-3 protein expression is shown as a bar chart (n = 6). Results are shown as mean ± SD. *** p < 0.001 versus the sham group; ### p < 0.001 versus the 2VO group.

Article Snippet: Prevotella histicola (DSMZ, Braunschweig, Germany) was fully dispersed into sterile water (10 10 cfu/g).

Techniques: Transplantation Assay, Expressing, Control

Journal: Brain Sciences

Article Title: Prevotella histicola Transplantation Ameliorates Cognitive Impairment and Decreases Oxidative Stress in Vascular Dementia Rats

doi: 10.3390/brainsci13081136

Figure Lengend Snippet: Prevotella histicola transplantation alleviates oxidative stress in hippocampi of VaD model rats. ( A ) PCC, ( B ) MDA levels, ( C ) SOD and ( D ) GPX activity in the hippocampi of the rats were measured based on the kit manufacturer’s instructions (n = 6 per group). Results are shown as mean ± SD. *** p < 0.001 versus the sham group; # p < 0.05, ## p < 0.01, ### p < 0.001 versus the 2VO group.

Article Snippet: Prevotella histicola (DSMZ, Braunschweig, Germany) was fully dispersed into sterile water (10 10 cfu/g).

Techniques: Transplantation Assay, Activity Assay

Journal: Brain Sciences

Article Title: Prevotella histicola Transplantation Ameliorates Cognitive Impairment and Decreases Oxidative Stress in Vascular Dementia Rats

doi: 10.3390/brainsci13081136

Figure Lengend Snippet: Prevotella histicola transplantation regulated the inflammatory cytokines after VaD. The mRNA levels of TNF-α ( A ), IL-1β ( B ) and IL-6 ( C ) in hippocampus tissue was detected by RT-qPCR (n = 6 per group). The protein levels of TNF-α ( D ), IL-1β ( E ) and IL-6 ( F ) in hippocampus tissue was detected by ELISA assay (n = 6 per group). Results are shown as mean ± SD. *** p < 0.001 versus the sham group; ### p < 0.001 versus the 2VO group.

Article Snippet: Prevotella histicola (DSMZ, Braunschweig, Germany) was fully dispersed into sterile water (10 10 cfu/g).

Techniques: Transplantation Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

Journal: Brain Sciences

Article Title: Prevotella histicola Transplantation Ameliorates Cognitive Impairment and Decreases Oxidative Stress in Vascular Dementia Rats

doi: 10.3390/brainsci13081136

Figure Lengend Snippet: Prevotella histicola transplantation regulates the activation of microglia and astrocytes and affects CaMKII phosphorylation in the hippocampi of VaD rats. ( A ) Representative bands of Iba-1 and GFAP protein expression within the hippocampi of the rats across the groups. β-actin served as a loading control (n = 6). Quantification for Iba-1 ( B ) and GFAP ( C ) protein expression is shown as a bar chart (n = 6). ( D ) Representative bands of CaMKII and phospho-CaMKII protein expression within the hippocampi of the rats across the groups. β-actin served as a loading control (n = 6). Quantification for CaMKII ( E ) and phospho-CaMKII ( F ) protein expression is shown as a bar chart (n = 6). Results are shown as mean ± SD. *** p < 0.001 versus the sham group; ## p < 0.01, ### p < 0.001 versus the 2VO group.

Article Snippet: Prevotella histicola (DSMZ, Braunschweig, Germany) was fully dispersed into sterile water (10 10 cfu/g).

Techniques: Transplantation Assay, Activation Assay, Phospho-proteomics, Expressing, Control